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Human Papillomavirus (HPV) DNA Detection with Genotyping, High Risk Types by PCR [A Test in Focus]

This new Mayo Clinic assay detects the 14 high-risk HPV types, and if present, provides specific genotype results for HPV-16 and/or HPV-18. Together, HPV types 16 and 18 cause more than 60 percent of cervical cancer in the United States. View a three-minute video interview with Dr. Matt Binnicker, which provides greater detail about this assay.

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  • Detection of high risk (HR) genotypes associated with the development of cervical cancer
  • An aid in triaging women with abnormal Pap smear results
  • Individual genotyping of human papillomavirus (HPV)-16 and/or HPV-18, if present
  • Results of HPV-16 and HPV-18 genotyping can be used as an aid in triaging women with positive HR-HPV but negative Pap smear results.
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When should I order this test?

  • This test may be ordered alongside cytology (i.e., co-testing) in women aged 30 to 65. In addition, this test may be used to assist in triaging women with abnormal (ASC-US) Pap smear results. Finally, the genotyping component of this test may be useful when co-testing reveals a positive result for high-risk HPV and normal cytology findings.

Highlights:

  • Up to 75% of women are exposed to human papilloma virus (HPV). Most infected women will clear the infection without any long-term health consequences.
  • Persistent HPV infection is the principal cause of cervical cancer and its precursor lesions (cervical intraepithelial neoplasia).
  • There are 14 HPV types (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) that are classified as high-risk for the development of cervical cancer and its precursor lesions.
  • HPV types 16 and 18 cause >60% of cervical cancer.
  • Testing by PCR has become a standard method for determining the presence of a cervical HPV infection
  • The Mayo Clinic assay detects the 14 high-risk HPV types, and if present, provides specific genotype results for HPV-16 and/or HPV-18.
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  • Real-Time Polymerase Chain Reaction (PCR) (PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

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  • Day(s) and Time(s) Test Performed: Monday through Friday; Varies
  • Analytic Time: 3 days
This entry was posted in Genitourinary / STI Infections, Microbiology / Infectious Disease, Test in Focus.