Human Papillomavirus (HPV) DNA Detection with Genotyping, High Risk Types by PCR [A Test in Focus]


This new Mayo Clinic assay detects the 14 high-risk HPV types, and if present, provides specific genotype results for HPV-16 and/or HPV-18. Together, HPV types 16 and 18 cause more than 60 percent of cervical cancer in the United States. View a three-minute video interview with Dr. Matt Binnicker, which provides greater detail about this assay.

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  • Detection of high risk (HR) genotypes associated with the development of cervical cancer
  • An aid in triaging women with abnormal Pap smear results
  • Individual genotyping of human papillomavirus (HPV)-16 and/or HPV-18, if present
  • Results of HPV-16 and HPV-18 genotyping can be used as an aid in triaging women with positive HR-HPV but negative Pap smear results.
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When should I order this test?

  • This test may be ordered alongside cytology (i.e., co-testing) in women aged 30 to 65. In addition, this test may be used to assist in triaging women with abnormal (ASC-US) Pap smear results. Finally, the genotyping component of this test may be useful when co-testing reveals a positive result for high-risk HPV and normal cytology findings.

Highlights:

  • Up to 75% of women are exposed to human papilloma virus (HPV). Most infected women will clear the infection without any long-term health consequences.
  • Persistent HPV infection is the principal cause of cervical cancer and its precursor lesions (cervical intraepithelial neoplasia).
  • There are 14 HPV types (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) that are classified as high-risk for the development of cervical cancer and its precursor lesions.
  • HPV types 16 and 18 cause >60% of cervical cancer.
  • Testing by PCR has become a standard method for determining the presence of a cervical HPV infection
  • The Mayo Clinic assay detects the 14 high-risk HPV types, and if present, provides specific genotype results for HPV-16 and/or HPV-18.
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  • Real-Time Polymerase Chain Reaction (PCR) (PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

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  • Day(s) and Time(s) Test Performed: Monday through Friday; Varies
  • Analytic Time: 3 days
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Andy Tofilon

Andy Tofilon is a Marketing Segment Manager at Mayo Medical Laboratories. He leads strategies for corporate communications, public relations, and new media innovations. Andy has worked at Mayo Clinic since 2003. Outside of work, Andy can be found running, hiking, snapping photos, and most importantly, spending time with his family.